Highlights

Validated for qualitative detection of SARS-CoV-2 (COVID-19)
Ultra-low sensitivity to low copy number templates
4X concentrated buffer enables more sample input volume
Contains the thermostable CelScript reverse transcriptase and novel Rnase inhibitor
Yields early Ct values with wide dynamic detection range
Compatible with TaqMan®, Scorpions® and molecular beacon probes
Enhanced specificity supported by a hot-start enzyme
Compatible with all qPCR cyclers

The CelEct Virus Universal Probe One-step kit is developed for fast and reproducible detection of viral RNA targets using probe-based real time one-step PCR. It can achieve sensitivity down to 4 copy inputs of RNA target and is compatible with probes based on various principles, including TaqMan®, Scorpions® and molecular beacon probes.

The 4X concentrated mix achieves ultra-low sensitivity by increasing the sample input volume and is ideal for high throughput assays with small reaction volumes. In addition to the advanced buffer composition, an RNase inhibitor protects viral RNA from degradation by RNases and complements the thermostable CelScript Reverse Transcriptase.

An antibody-mediated hot-start polymerase limits the formation of primer dimers and other non-specific products leading to efficient amplification of multiple targets, even in the presence of common inhibitors found in clinical samples.

The qPCR mix is supplied with a separate tube of 50μM ROX dye that may be used with instruments requiring ROX as a passive reference dye. CelProbe Universal qPCR mix is suitable for use on all real time PCR cyclers.

Use restrictions

For research use only. Not for use in diagnostic procedures.

Qualitative detection of SARS-CoV-2/COVID-19
Efficient amplification in single- and multiplex reactions
Ultra-sensitive detection of RNA viruses
Diagnostic qPCR assays
Detection of low copy number targets

Instrument compatibility	For use with all real time PCR instruments (supplied with a separate tube of rox dye)
Amplification detection	TaqMan®, Scorpions® and molecular beacon probes
Format	Ready-to-use one-step mix (4X)
Polymerase	CelTaq HS DNA polymerase
Reverse Transcriptase	CelScript Reverse Transcriptase
Increased specificity	Hot-start modification
Cycling speed	Standard and fast modes
Shelf life	12 months (if stored correctly)

Cat no.	Pack size	4X CelEct Virus Universal Probe One-step mix (no-ROX)	20X CelScript Reverse Transcriptase	ROX Additive (50µM)
CM3711-0200	200 rxns	1 x 1ml	1 x 0.2ml	1 x 0.2ml
CM3711-0600	600 rxns	3 x 1ml	1 x 0.6ml	1 x 0.2ml
CM3711-0600	1000 rxns	1 x 5ml	1 x 1ml	1 x 0.2ml

CelEct Virus Universal Probe One-step kit Product Brochure

CelEct Virus Universal Probe One-step kit Product Manual

CelEct Virus Universal Probe One-step kit Product Manual -mobile

CelEct Virus Universal Probe One-step kit MSDS

Store the kit at -20°C upon arrival. Full enzyme activity is retained for 12 months when stored at the recommended conditions and can endure up to 30 freeze/thaw cycles. Limit direct light exposure for extended periods.