CelTaq HS DNA Polymerase - sample

Hot start DNA Polymerase with enhanced specificity

CM2311-0050

R 87.21 ZAR

Highlights

Reduced non-specific amplification and primer dimers
Room temperature reaction set-up with increased yield and specificity
Efficient amplification of up to 6kb amplicons
Balanced salt solution with proprietary enhancers and stabilizers eliminates the need for buffer optimization
Superior performance on high GC/AT templates

CelTaq DNA Polymerase is a reliable, cost-effective solution for everyday PCR. This robust enzyme tolerates PCR inhibitors and gives consistent amplification of difficult templates (egg. mammalian genomic DNA that is rich in GC and AT content). A 12-step purification process eliminates residual host DNA resulting in ultra-low background, while effectively amplifying targets present in low copy numbers.

The enhanced 5x buffer system contains 5mM dNTPs, 15mM MgCl2, stabilizers and enhancers and does not require additional optimization. The system produces high yields under standard and fast cycling conditions to efficiently amplify targets up to 6kb in length. PCR products contains a A-tail at the 3’ end and can be used in TA cloning.

The enzyme is stable at room temperature for 4 weeks without affecting the product performance.

Use restrictions

For research use only. Not for use in diagnostic procedures.

Difficult templates
Routine PCR and multiplexing
High throughput screening with extended reaction set-up
Low copy template detection
TA-cloning
Genotyping
Colony PCR
Direct PCR (urine, blood, bacterial cultures)

Format	Enzyme and buffer separate
Polymerase concentration	5U/µl
Buffer concentration	5x
Direct gel-loading	No
Error rate	1 error per 2.0 x 105 nucleotides incorporated
Increased specificity	Antibody-mediated hot start
Fidelity (vs. Taq DNA polymerase)	1x
Proofreading activity	No
Exonuclease activities	5'-3' exonuclease activities
Amplicon range	Up to 6kb
Product 3' ends	A-tailed
Shelf life	12 months (if stored correctly)

Cat no.	CM2311-0250	CM2311-1000
Pack size	250 units	1000 units
CelTaq HS DNA polymerase, 5U/µl	1 x 0.05ml	4 x 0.05ml
5x CelTaq HS buffer	2 x 1ml	8 x 1ml

CelTaq HS DNA Polymerase Product Brochure

CelTaq HS DNA Polymerase Product Manual

CelTaq HS DNA Polymerase Product Manual – mobile

CelTaq HS DNA Polymerase MSDS

Store the kit at -20°C upon arrival. This product is shipped on ice blocks and can be kept at 4°C for 4 weeks without affecting the product performance. Full enzyme activity is retained for 12 months when stored at the recommended conditions and can endure up to 30 freeze/thaw cycles.